Dr Tracey Gloster

Wellcome Trust Research Fellow

BMS B209
tel. 01334 467245
fax. 01334 462595

I am interested in the structure and function of eukaryotic carbohydrate processing enzymes. Obtaining a greater appreciation of how these enzymes assimilate or degrade carbohydrates is important in understanding the biological roles that carbohydrates play in cellular processes, and also how these processes malfunction in a number of diseases, such as cancer, lysosomal storage disorders, and neurodegenerative diseases.

I am currently investigating the enzymes responsible for the degradation of heparan sulphate, a component of the extracellular matrix. Using a combination of approaches, including molecular biology, protein production, X-ray crystallography, enzyme kinetics and cell culture, I aim to gain a greater understanding of these enzymes from a mechanistic and structural perspective. Hopefully this will lead to the development of tools, such as enzyme inhibitors, that can be used to probe the biological functions of the enzymes, and possibly also for therapeutic applications in the longer term.



Liu, T, Zandberg, WF, Gloster, TM, Deng, L, Murray, KD, Shan, X & Vocadlo, DJ 2018, 'Metabolic inhibitors of O-GlcNAc transferase (OGT) that act in vivo implicate decreased O-GlcNAc levels in leptin-mediated nutrient sensing' Angewandte Chemie International Edition, vol In press. DOI: 10.1002/anie.201803254
Adamson, C, Pengelly, RJ, Kazem Abadi, SS, Chakladar, S, Draper, J, Britton, R, Gloster, TM & Bennet, AJ 2016, 'Structural snapshots for mechanism-based inactivation of a glycoside hydrolase by cyclopropyl-carbasugars' Angewandte Chemie International Edition, vol 55, no. 48, pp. 14978-14982. DOI: 10.1002/anie.201607431
Alteen, MG, Oehler, V, Nemčovičová, I, Wilson, IBH, Vacadlo, DJ & Gloster, TM 2016, 'Mechanism of the human nucleocytoplasmic hexosaminidase D' Biochemistry, vol 55, no. 19, pp. 2753-2747. DOI: 10.1021/acs.biochem.5b01285
Gloster, TM 2014, 'Advances in understanding glycosyltransferases from a structural perspective' Current Opinion in Structural Biology, vol 28, pp. 131-141. DOI: 10.1016/j.sbi.2014.08.012
He, X, Pierce, O, Haselhorst, T, Kolarich, D, Packer, NH, Gloster, T, Vocadlo, DJ, Qian, Y, Brooks, D & Kermode, AR 2013, 'Characterization and downstream mannose phosphorylation of human recombinant α-L-iduronidase produced in Arabidopsis complex glycan-deficient (cgl) seeds' Plant Biotechnology Journal, vol 11, no. 9, pp. 1034–1043. DOI: 10.1111/pbi.12096
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