Dr Shirley Graham

Research Technician

BMS B313 Annexe
tel. 01334 463419/3436
fax. 01334 462595
sg71@st-andrews.ac.uk

Selected Publications

  1. Ring nucleases deactivate type III CRISPR ribonucleases by degrading cyclic oligoadenylate. Athukoralage JS, Rouillon C, Graham S, Grüschow S, White MF Nature 561(772) Oct 2018
  2. Control of cyclic oligoadenylate synthesis in a type III CRISPR system. Rouillon C, Athukoralage JS, Graham S, Grüschow S, White MF eLife Sciences 7 Jul 2018.
  3. Prespacer processing and specific integration in a Type I-A CRISPR system. Rollie C, Graham S, Rouillon C, White MF. Nucleic Acids Research 46(3) Dec 2017
  4. Multiple nucleic acid cleavage modes in divergent type III CRISPR systems. Zhang J, Graham S, Tello A, Liu H, White MF. Nucleic Acids Research 44(4). Jan 2016
  5. Cas6 specificity and CRISPR RNA loading in a complex CRISPR-Cas system. Sokolowski RD, Graham S, White MF, Nucleic Acids Research 42(10) Apr 2014.
  6. Structure of the CRISPR Type IIIA Effector Complex: conservation of form and function with type I systems. Rouillon C, Zhou M, Zhang J, Politis A, Beilsten V, Cannone G, Graham S, Robinson CV, Spagnolo L, White MF. Molecular Cell 52(1) Oct 2013.
  7. MRSA decolonization of cotton rat nares by a combination treatment comprising lysostaphin and the antimicrobial peptide ranalexin. Desbois AP, Sattar A, Graham S, Warn PA, Coote PJ. Journal of Antimicrobial Chemotherapy 68(11) Jun 2013
  8. Structure of the archaeal Cascade subunit Csa5: Relating the small subunits of CRISPR effector complexes. Reeks J, Graham S, Anderson L, Liu H, White MF, Naismith JH. RNA biology 10(5) Apr 2013.
  9. Structure of a dimeric crenarchaeal Cas6 enzyme with an atypical active site for CRISPR RNA processing. Reeks J, Sokolowski RD, Graham S, Liu H, Naismith JH, White MF. Biochemical Journal 452(2) Mar 2013.
  10. A model for 3-methyladenine recognition by 3-methyladenine DNA glycosylase I (TAG) from Staphylococcus aureus Zhu X, Yan X, Carter LG, Liu H, Graham S, Coote PJ, Naismith J. Acta Crystallogr Sect F Struct Biol Cryst Commun. 68(6) Jun 2012.
  11. Structure and mechanism of the CMR complex for CRISPR-Mediated antiviral immunity. Zhang J, Rouillon C, Kerou M, Reeks J, Brugger K, Graham S, Reimann J, Cannone G, Liu H, Albers SV, Naismith JH, Spagnolo L, White MF. Molecular Cell 45(3) Feb 2012. 
  12. Displacement of the canonical single-stranded DNA-binding protein in the Thermoproteales. Paytubi S, McMahon SA, Graham S, Liu H, Botting CH, Makarova KS, Koonin EV, Naismith JH, White MF. Proceedings of the National Academy of Sciences 109(7) Nov 2011.
  13. Structural and Functional Characterization of an Archaeal Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated Complex for Antiviral Defense (CASCADE).  Lintner NG, Kerou M, Brumfield SK, Graham S, Liu H, Naismith JH, Sdano M, Peng N, She Q, Copie V, Young MJ, White MF, Lawrence CM. Journal of Biological Chemistry 286(24) Jun 2011.
  14. Global Network Analysis of Drug Tolerance, Mode of Action and Virulence in Methicillin-Resistant S. aureus.  Overton IM, Graham S, Gould KA, Hinds J, Botting CH, Shirran S, Barton GJ, Coote PJ.  BMC Systems Biol. 68(5) May 2011
  15. Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.  Seetharamappa J, Oke M, Liu H, McMahon SA, Johnson KA, Carter L, Dorward M, Zawadzki M, Overton IM, van Niekirk CA, Graham S, Botting CH, Taylor GL, White MF, Barton GJ, Coote PJ, Naismith JH. Acta Crystallogr Sect F Struct Biol Cryst Commun. 63(6) Jun 2007.
  16. Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/ tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase. Seetharamappa J, Oke M, Liu H, McMahon SA, Johnson KA, Carter L, Dorward M, Zawadzki M, Overton IM, van Niekirk CA, Graham S, Botting CH, Taylor GL, White MF, Barton GJ, Coote PJ, Naismith JH. Acta Crystallogr Sect F Struct Biol Cryst Commun. 63(5) May 2007
  17. Potent, synergistic inhibition of Staphylococcus aureus upon exposure to a combination of the endopeptidase lysostaphin and the cationic peptide ranalexin.  Graham S, Coote PJ. J Antimicrob Chemother. 59(4) Apr 2007
(source: symbiosis database)