& Smith, VJ
Disk Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts
Natural Products From Marine Algae: Marine algae are a relatively untapped source of bioactive natural products, including those with antimicrobial activities. The ability to assess the antimicrobial activity of cell extracts derived from algal cultures is vital to identifying species that may produce useful novel antibiotics. One assay that is used widely for this purpose is the disk diffusion assay due to its simplicity, rapidity, and low cost. Moreover, this assay gives output data that are easy to interpret and can be used to screen many samples at once irrespective of the solvent used during preparation. In this chapter, a step-by-step protocol for performing a disk diffusion assay is described. The assay is particularly well suited to testing algal cell extracts and fractions resulting from separation through bioassay-guided approaches..
vol. 1308, Springer, pp. 403.
Robb, C, Dyrynda, E, Gray, R, Rossi, A
& Smith, VJ
Invertebrate extracellular phagocyte traps show that chromatin is an ancient defence weapon
, vol 5, 4627.
Andrews, PLR, Darmaillacq, A-S, Dennison, N, Gleadall, IG, Hawkins, P, Messenger, JB, Osorio, D
, Smith, VJ
& Smith, JA 2013, '
The identification and management of pain, suffering and distress in cephalopods, including anaesthesia, analgesia and humane killing
Journal of Experimental Marine Biology and Ecology
, vol 447, pp. 46-64.
Phylogeny of whey acidic protein (WAP) four-disulfide core proteins and their role in lower vertebrates and invertebrates
Biochemical Society Transactions
, vol 39, no. 5, pp. 1403-1408.
, Roulston, C & Dyrynda, EA 2010, '
The Shrimp Immune System
'. in V Alday-Sanz (ed.),
The Shrimp Book.
1 edn, Nottingham University Press, Nottingham UK, pp. 89-148.