{"id":25,"date":"2020-04-21T11:42:11","date_gmt":"2020-04-21T11:42:11","guid":{"rendered":"https:\/\/synergy.st-andrews.ac.uk\/cob2\/?page_id=25"},"modified":"2023-10-07T16:31:21","modified_gmt":"2023-10-07T15:31:21","slug":"facilities","status":"publish","type":"page","link":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/facilities\/","title":{"rendered":"Facilities"},"content":{"rendered":"\n

We have multiple light microscopes and workstations dedicated to image processing. Some of our microscopes are commercial instruments but a significant number of them are innovative techniques developed by groups within the Centre, and therefore, are custom-built setups.  Our imaging facilities are additionally complemented by nano- and microfabrication techniques within a cleanroom environment and ultra-fast techniques including femtosecond spectroscopy, fluorescence up-conversion and transient-absorption spectroscopy. This provides a broad experimental platform to address important biological questions across temporal and spatial scales and drive innovation. Below, we list some of our instruments and facilities. If you are interested in having more information as a potential user please contact us.<\/p>\n\n\n\n

Equipment – Commercial light microscopes<\/h2>\n\n\n\n
\n
\n

ZEISS AiryScan <\/span><\/h3>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: BSRC  Level 1, School of Biology, Contact: Dr Marcus Bischoff<\/p>\n<\/div>\n\n\n\n

\n

LEICA TCS SP8<\/span><\/h3>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: Room B122, Biomedical Science Research Complex (BSRC), School of Biology.<\/p>\n<\/div>\n\n\n\n

\n

ZEISS Axioplan 2<\/span><\/h3>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: Room B122, Biomedical Science Research Complex (BSRC), School of Biology.<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n
\n

ZEISS Pascal 510 <\/span>confocal<\/h3>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: Room B106, Biomedical Science Research Complex (BSRC), School of Biology.<\/p>\n<\/div>\n\n\n\n

\n

ZEISS A<\/span>potome<\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: School of Psychology and Neuroscience<\/p>\n<\/div>\n\n\n\n

\n

Crest X-light confocal<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: School of Psychology and Neuroscience<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n
\n

S<\/span>pinning disk confocal<\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: Wolfson laboratories. School of Medicine.<\/p>\n<\/div>\n\n\n\n

\n

NIKON N-SIM<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location:  School of Physics and Astronomy<\/em><\/span><\/p>\n<\/div>\n\n\n\n

\n

ZEISS Axioscan Z1<\/span><\/h4>\n\n\n\n

\"\"<\/span><\/strong><\/h3>\n\n\n\n

<\/p>\n\n\n\n

Location:  School of Medicine<\/em><\/span><\/p>\n<\/div>\n<\/div>\n\n\n\n

\n
\n

D<\/span>eltavision<\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: ROOM B104, Biomedical Science Research Complex (BSRC), School of Biology.<\/p>\n<\/div>\n\n\n\n

\n

ZEISS Axiovert – M<\/span>icroinjection<\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location:\u00a0 School of Physics and Astronomy<\/p>\n<\/div>\n\n\n\n

\n

LEICA LMD6500<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location:\u00a0 School of Biology, BSRC Annex<\/p>\n<\/div>\n<\/div>\n\n\n\n

Equipment – Custom-built microscopes<\/h2>\n\n\n\n
\n
\n

Total-Internal Reflection (TIR) <\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: Prism-type TIR Level 1 , Biomedical Science Research Complex (BSRC), School of Biology. Objective-type TIR: <\/em><\/p>\n<\/div>\n\n\n\n

\n

Single-molecule FRET<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: Level 1, Biomedical Science Research Complex (BSRC), School of Biology.<\/p>\n<\/div>\n\n\n\n

\n

Single-molecule spectrograph<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: Room 268, School of Physics and Astronomy<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n
\n

Single-molecule FCS-MFD<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: Biomedical Science Research Complex (BSRC), School of Biology.<\/p>\n<\/div>\n\n\n\n

\n

Combined magnetic tweezers and fluorescence imaging<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location: Rom 268, School of Physics and Astronomy<\/p>\n<\/div>\n\n\n\n

\n

TRAFIX multiphoton microscope<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

TRAFIX: temporal focussing with single pixel detection for imaging at depth<\/em>. <\/p>\n\n\n\n

Location:\u00a0 School of Physics and Astronomy<\/p>\n<\/div>\n<\/div>\n\n\n\n

\n
\n

Custom light sheet microscopes<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location:\u00a0 School of Physics and Astronomy<\/p>\n<\/div>\n\n\n\n

\n

Raman\/holographic microscopy<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location:\u00a0 School of Physics and Astronomy<\/p>\n<\/div>\n\n\n\n

\n

Acoustic trapping\/Raman spectroscopy platform<\/span><\/h4>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Location:\u00a0 School of Physics and Astronomy<\/p>\n<\/div>\n<\/div>\n\n\n\n

Femtosecond spectroscopy facilities<\/h2>\n\n\n\n
\"\"<\/figure>\n\n\n\n

We have access to a number of instruments for femtosecond spectroscopy including:<\/p>\n\n\n\n

    \n
  • Femtosecond lasers<\/strong> (SpectraPhysics Mai Tai Ti:Sapphire oscillator – 80 MHz output, 750-850 nm, 100 fs FWHM pulse width, SpectraPhysics Merlin laser at 527 nm, 50 KHz and 200 ns pulse width)<\/li>\n\n\n\n
  • Light amplifiers<\/strong> (SpectraPhysics Hurricane, SpectraPhysics OPA, Light-Conversion TOPAS-White and SpectraPhysics Spitfire.<\/li>\n\n\n\n
  • Hamamatsu Streak Camera<\/strong>\u00a0<\/span>\u2013 Detection of luminescence from thin films or solutions, with an instrument response function of ~ 2 ps (FWHM).<\/li>\n\n\n\n
  • CDP FOG 100<\/strong>\u00a0<\/span>\u2013 Optical gating allows luminescence to be detected on the femtosecond timescale. By sum-frequency mixing of the gating pulse and sample luminescence, upconverted light is produced that is detected. This technique allows temporal luminescence kintetics with time constants as low as 50 fs to be resolved.<\/li>\n\n\n\n
  • Homebuilt Transient Absorption<\/strong>\u00a0<\/span>\u2013 Utilising two beams, a pump and a probe, with one of them delayable, allows transient kinetics in the absorption of samples to be recorded on the femtosecond timescale.<\/li>\n\n\n\n
  • These facilities are hosted at the School of Physics and Astronomy and coordinated by the labs of Prof . Samuel and Prof. Turnbull. (Image courtesy of Ifor Samuel\/Graham Turnbull)<\/li>\n<\/ul>\n\n\n\n
    \n
    \n

    Nanofabrication facilities<\/h2>\n\n\n\n
    \n
    \n
    \"\"<\/figure>\n<\/div>\n\n\n\n
    \n
    \"\"<\/figure>\n<\/div>\n<\/div>\n\n\n\n

    We have facilities to manufacture micro- and nano-patterned photonics structures via electron-beam lithography and to characterize them via AFM, 3D microprinting, DEKTAK profiler and EM. If necessary, the fabrication process can be carried out in a cleanroom facility and entirely in a nitrogen atmosphere glovebox. These facilities are hosted in the School of Physics and Astronomy and managed by Prof Samuel, Prof Turnbull, Prof. Malte Gather and Prof di Falco.\u00a0In addition, instrument developers at the CoB have access to excellent electronic and mechanical workshops to assist the researchers in the design, drawing and manufacturing process of specialized components. (Image courtesy Andrea di Falco).<\/p>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

    We have multiple light microscopes and workstations dedicated to image processing. Some of our microscopes are commercial instruments but a significant number of them are innovative techniques developed by groups within the Centre, and therefore, are custom-built setups.  Our imaging facilities are additionally complemented by nano- and microfabrication techniques within a cleanroom environment and ultra-fast…<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"full-width-page.php","meta":{"footnotes":""},"class_list":["post-25","page","type-page","status-publish","hentry"],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/pages\/25","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/comments?post=25"}],"version-history":[{"count":0,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/pages\/25\/revisions"}],"wp:attachment":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/media?parent=25"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}