{"id":31553,"date":"2021-04-14T11:06:46","date_gmt":"2021-04-14T11:06:46","guid":{"rendered":"http:\/\/synergy.st-andrews.ac.uk\/cob\/?p=31553"},"modified":"2021-04-14T11:06:46","modified_gmt":"2021-04-14T11:06:46","slug":"fernando-herreo","status":"publish","type":"post","link":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/2021\/04\/14\/fernando-herreo\/","title":{"rendered":"CTP promotes efficient ParB-dependent DNA condensation by facilitating 1D spreading from parS"},"content":{"rendered":"<h3><em>Prof Fernando Moreno-Herrero<\/em><em>, Centro Nacional de Biotecnologia, CSIC, Madrid, Spain<\/em><\/h3>\n<h3><strong><em>28th April 2021, 1.00pm, <\/em><\/strong><strong><em>Microsoft Teams<\/em><\/strong><\/h3>\n<h1><strong>CTP promotes efficient ParB-dependent DNA condensation by facilitating 1D spreading from <em>parS<\/em><\/strong><\/h1>\n<p style=\"text-align: justify\">Faithful segregation of bacterial chromosomes relies on the ParABS partitioning system and the SMC complex. The ParABS system consists of the ATPase protein ParA, the DNA-binding protein ParB, and a centromere-like palindromic DNA sequence named <em>parS<\/em>. In this seminar, first, I will give you an overview of our previous studies regarding the interaction of ParB with DNA. Then, motivated by the recent finding that ParB is a CTPase, I will focus on our latest work that investigate the role of cytidine triphosphate (CTP) binding and hydrolysis in the critical interaction between <em>parS<\/em> DNA sequences and ParB. We have employed a combined optical tweezers confocal microscope to directly observe the specific interaction of ParB with <em>parS<\/em> and non-cognate sequences, and magnetic tweezers to reveal an efficient DNA condensation at nanomolar ParB concentrations.<\/p>\n<p><a href=\"http:\/\/www.fernandomorenoherrero.com\/\">http:\/\/www.fernandomorenoherrero.com\/\u00a0<\/a><\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Prof Fernando Moreno-Herrero, Centro Nacional de Biotecnologia, CSIC, Madrid, Spain 28th April 2021, 1.00pm, Microsoft Teams CTP promotes efficient ParB-dependent DNA condensation by facilitating 1D spreading from parS Faithful segregation of bacterial chromosomes relies on the ParABS partitioning system and the SMC complex. The ParABS system consists of the ATPase protein ParA, the DNA-binding protein&hellip;<\/p>\n","protected":false},"author":29,"featured_media":31554,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[7],"tags":[],"class_list":["post-31553","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-seminars"],"jetpack_featured_media_url":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-content\/uploads\/sites\/13\/2021\/04\/Fernando-Herrero-Moreno.png","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/posts\/31553","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/users\/29"}],"replies":[{"embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/comments?post=31553"}],"version-history":[{"count":0,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/posts\/31553\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/media\/31554"}],"wp:attachment":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/media?parent=31553"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/categories?post=31553"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/tags?post=31553"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}