{"id":32193,"date":"2024-04-23T21:34:50","date_gmt":"2024-04-23T20:34:50","guid":{"rendered":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/?p=32193"},"modified":"2024-04-23T21:39:05","modified_gmt":"2024-04-23T20:39:05","slug":"imaging-protein-nucleic-acid-interactions-across-scales-and-with-single-molecule-resolution","status":"publish","type":"post","link":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/2024\/04\/23\/imaging-protein-nucleic-acid-interactions-across-scales-and-with-single-molecule-resolution\/","title":{"rendered":"Imaging Protein-Nucleic Acid Interactions Across Scales and with Single Molecule Resolution"},"content":{"rendered":"\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-9d6595d7 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<div class=\"wp-block-media-text is-stacked-on-mobile\" style=\"grid-template-columns:22% auto\"><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"187\" height=\"251\" src=\"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-content\/uploads\/sites\/13\/2024\/04\/mrc-lms-0353_1633948467979_x1-1.jpg\" alt=\"\" class=\"wp-image-32195 size-full\" \/><\/figure><div class=\"wp-block-media-text__content\">\n<p><strong>Speaker: Prof David Rueda, Imperial College London<\/strong>. <\/p>\n\n\n\n<p>Faculty of Medicine,&nbsp;Department of Infectious Disease <\/p>\n\n\n\n<p><em>Chair in Molecular and Cellular Biophysic<\/em>s<\/p>\n<\/div><\/div>\n<\/div>\n<\/div>\n\n\n\n<p>Host: Prof Carlos Penedo<\/p>\n\n\n\n<p><strong>1st May 2024, 1 pm, BMS Seminar Room<\/strong><\/p>\n\n\n\n<p>Over the past decade, the market in RNA-based therapeutics and technology has&nbsp;emerged rapidly in the field of life sciences, experiencing a sizeable compound&nbsp;annual growth rate. Examples of early FDA-approved RNA-based drugs are&nbsp;spinraza, an antisense oligonucleotide developed to treat spinal muscular atrophy by&nbsp;regulating mRNA splicing, and patisiran, based on RNA interference to treat hereditary transthyretin-mediated amyloidosis. Other key RNA-based technologies are CRISPR\/Cas9 for gene editing and, most recently, the latest RNA vaccines by&nbsp;Pfizer and Moderna to treat COVID-19.&nbsp;<\/p>\n\n\n\n<p>To understand how RNA therapeutics function and to further develop new RNA-based drugs, it is essential to elucidate their mechanism at the molecular level.&nbsp;Single-molecule microscopy approaches provide unique opportunities to investigate fundamental biological processes involving nucleic acids and proteins. By eliminating ensemble-averaging, they enable monitoring such processes in real-time, providing an opportunity to track dynamic events, molecular interactions, and the formation of&nbsp;large macromolecular complexes and transient biomolecular species with&nbsp;unprecedented resolution.<\/p>\n\n\n\n<p>Our laboratory develops and applies fluorescence- and\u00a0force-based single-molecule microscopy approaches to study fundamental biological\u00a0processes involving RNA molecules and RNA-protein complexes.\u00a0In this presentation, we will present our most recent efforts to determine the\u00a0molecular factors that lead to CRISPR\/Cas9 off-target activity and our recent\u00a0advances using fluorogenic RNA aptamers for background-free imaging of cellular\u00a0RNAs with single-molecule resolution.\u00a0<\/p>\n\n\n\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"480\" height=\"360\" src=\"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-content\/uploads\/sites\/13\/2024\/04\/hqdefault-1.jpg\" alt=\"\" class=\"wp-image-32198\" \/><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>Speaker: Prof David Rueda, Imperial College London. Faculty of Medicine,&nbsp;Department of Infectious Disease Chair in Molecular and Cellular Biophysics Host: Prof Carlos Penedo 1st May 2024, 1 pm, BMS Seminar Room Over the past decade, the market in RNA-based therapeutics and technology has&nbsp;emerged rapidly in the field of life sciences, experiencing a sizeable compound&nbsp;annual growth&hellip;<\/p>\n","protected":false},"author":29,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[7],"tags":[],"class_list":["post-32193","post","type-post","status-publish","format-standard","hentry","category-seminars"],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/posts\/32193","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/users\/29"}],"replies":[{"embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/comments?post=32193"}],"version-history":[{"count":0,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/posts\/32193\/revisions"}],"wp:attachment":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/media?parent=32193"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/categories?post=32193"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/tags?post=32193"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}