{"id":531,"date":"2020-05-16T12:55:21","date_gmt":"2020-05-16T12:55:21","guid":{"rendered":"https:\/\/synergy.st-andrews.ac.uk\/cob2\/?p=531"},"modified":"2020-05-16T12:55:21","modified_gmt":"2020-05-16T12:55:21","slug":"single-molecule-fret-unveils-ubiquitin-transfer-mechanism","status":"publish","type":"post","link":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/2020\/05\/16\/single-molecule-fret-unveils-ubiquitin-transfer-mechanism\/","title":{"rendered":"Single molecule FRET unveils ubiquitin transfer mechanism"},"content":{"rendered":"

Single molecule FRET unveils ubiquitin transfer mechanism<\/h1>\n

We use single molecule F\u00f6rster Resonance Energy Transfer (smFRET) to measure the conformation of a FRET labelled E2~Ub conjugate, which distinguishes between closed and alternative conformations. We describe a real-time FRET assay with a thioester linked E2~Ub conjugate to monitor single ubiquitination events and demonstrate that ubiquitin is transferred to substrate from the closed conformation. These findings are likely to be relevant to all RING E3 catalysed reactions ligating ubiquitin and other ubiquitin-like proteins (Ubls) to substrates.<\/p>\n

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Branigan, E. J. C. P. R. T. H. Ubiquitin transfer by a RING E3 ligase occurs from a closed E2~ubiquitin conformation. Nature Communications<\/i> 11 (2020).<\/a><\/div>\n<\/div>\n

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The Laboratory for Biophysics and Biomolecular Dynamics incollaboration with Prof. R. Hay and Dr. Emma Branigan at Dundee University, demonstrates using smFRET that ubiquitin transfer by RING E3 ligases occurs from a close conformation of the E2~Ub complex. The work has just been published in Nature Communications volume 11, Article number: 2846 (2020)<\/p>\n","protected":false},"author":29,"featured_media":532,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[6],"tags":[],"class_list":["post-531","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-news"],"jetpack_featured_media_url":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-content\/uploads\/sites\/13\/2020\/05\/Ubiquitin-1.png","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/posts\/531","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/users\/29"}],"replies":[{"embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/comments?post=531"}],"version-history":[{"count":0,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/posts\/531\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/media\/532"}],"wp:attachment":[{"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/media?parent=531"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/categories?post=531"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/biology.st-andrews.ac.uk\/biophotonics\/wp-json\/wp\/v2\/tags?post=531"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}