Jens Tilsner

jt58
Thursday 1 December 2022


Research Centre:



Group Highlights


Our group investigates the structure and function of nano-channels connecting plant cells (plasmodesmata), and how these are hijacked by plant viruses to spread through the host.
We use molecular biology, biochemistry, structural biology, live cell and super-resolution imaging, electron microscopy, reverse and forward genetics, and transient and stable plant transformation to address these questions.



Research


BBSRC Pathfinder Impact Acceleration Account “Pathways on a Particle” (2022-2023), collaboration with Prof Rebecca Goss, School of Chemistry

BBSRC EASTBIO PhD project “Regulation of Plant Cell-to-Cell Communication” (2020-2024), collaboration with Dr Piers Hemsley (University of Dundee) and Dr Alison Roberts (James Hutton Institute)

BBSRC Responsive Mode project “Push on through to the other side – molecular basis of viral cell-to-cell movement in plants” (2015-2019)

Group Members


David Burnett, PhD student/PDRA
Zoe Barr, PhD student

Publications

Chapters

Cell-to-cell connectivity assays for the analysis of cytoskeletal and other regulators of plasmodesmata
Barr, Z. & Tilsner, J., 12 Feb 2023, The plant cytoskeleton: methods and protocols. Hussey, P. J. & Wang, P. (eds.). New York, NY: Humana Press, p. 193-202 10 p. (Methods in molecular biology; vol. 2604).
Research output: Chapter in Book/Report/Conference proceedingChapter

RNA imaging with RNase-inactivated Csy4 in plants and filamentous fungi
Burnett, D., Lichius, A. & Tilsner, J., 2020, RNA Tagging: Methods and Protocols. Heinlein, M. (ed.). Springer, (Methods in Molecular Biology; vol. 2166).
Research output: Chapter in Book/Report/Conference proceedingChapter

Growing uses of 2A in Plant Biotechnology
Luke, G. A., Roulston, C., Tilsner, J. & Ryan, M. D., 2015, Biotechnology. Ekinci, D. (ed.). Croatia: InTech, p. 165-193 28 p.
Research output: Chapter in Book/Report/Conference proceedingChapter

 

Articles

Heavy metal-associated isoprenylated plant proteins (HIPPs) at plasmodesmata: exploring the link between localization and function
Barr, Z. K., Werner, T. & Tilsner, J., 21 Aug 2023, In: Plants. 12, 16, 15 p., 3015.
Research output: Contribution to journalReview articlepeer-review

Tomato SlGSTU38 interacts with the PepMV coat protein and promotes viral infection
Méndez-López, E., Donaire, L., Gosálvez, B., Díaz-Vivancos, P., Sánchez-Pina, M. A., Tilsner, J. & Aranda, M. A., 1 Apr 2023, In: New Phytologist. 238, 1, p. 332-348 17 p.
Research output: Contribution to journalArticlepeer-review

Reticulons 3 and 6 interact with viral movement proteins
Tilsner, J. & Kriechbaumer, V., 20 Aug 2022, (E-pub ahead of print) In: Molecular Plant Pathology. Early View, 8 p.
Research output: Contribution to journalArticlepeer-review

Subcellular localization and interactions among TGB proteins of cowpea mild mottle virus
Carvalho, S. L., Tilsner, J., Figueira, A. R. & Carvalho, C. M., 1 Dec 2022, In: Archives of Virology. 167, p. 2555-2566 12 p.
Research output: Contribution to journalArticlepeer-review

Creating contacts between replication and movement at plasmodesmata – a role for membrane contact sites in plant virus infections?
Levy, A. & Tilsner, J., 3 Jul 2020, In: Frontiers in Plant Science. 11, 8 p., 862.
Research output: Contribution to journalReview articlepeer-review

Multiple C2 domains and Transmembrane region Proteins (MCTPs) tether membranes at plasmodesmata
Brault, M. L., Petit, J. D., Immel, F., Nicolas, W. J., Glavier, M., Brocard, L., Gaston, A., Fouché, M., Hawkins, T. J., Crowet, J-M., Grison, M. S., Germain, V., Rocher, M., Kraner, M., Alva, V., Claverol, S., Paterlini, A., Helariutta, Y., Deleu, M., Lins, L., & 2 othersTilsner, J. & Bayer, E. M., 1 Aug 2019, In: EMBO Reports. 20, 8, 26 p., e47182.
Research output: Contribution to journalArticlepeer-review

Efficient detection of long dsRNA in vitro and in vivo using the dsRNA binding domain from FHV B2 protein
Monsion, B., Incarbone, M., Hleibieh, K., Poignavent, V., Ghannam, A., Dunoyer, P., Daeffler, L., Tilsner, J. & Ritzenthaler, C., 1 Feb 2018, In: Frontiers in Plant Science. 9, 16 p., 70.
Research output: Contribution to journalArticlepeer-review

Hitchhikers, highway tolls and roadworks: the interactions of plant viruses with the phloem
Folimonova, S. Y. & Tilsner, J., Jun 2018, In: Current Opinion in Plant Biology. 43, p. 82-88 7 p.
Research output: Contribution to journalReview articlepeer-review

Architecture and permeability of post-cytokinesis plasmodesmata lacking cytoplasmic sleeves
Nicolas, W. J., Grison, M. S., Trépout, S., Gaston, A., Fouché, M., Cordelières, F. P., Oparka, K., Tilsner, J., Brocard, L. & Bayer, E. M., 1 Jul 2017, In: Nature Plants. 3, 7, 17082.
Research output: Contribution to journalArticlepeer-review

The potential consequences for cell Signaling by a class of NOD-Like Receptor proteins (NLRs) bearing an N-terminal signal sequence
Ryan, M. D., Roulston, C., de Felipe, P., Odon, V., Tilsner, J. & Luke, G. A., 19 May 2017, (E-pub ahead of print) In: Journal of Cell Signaling. 2, 2, 3 p., 148.
Research output: Contribution to journalArticlepeer-review

 

Other publications

Specialized membrane domains of plasmodesmata, plant intercellular nanopores
Bayer, E. M., Mongrand, S. & Tilsner, J., 30 Sept 2014, In: Frontiers in Plant Science. 5, 3 p., 507.
Research output: Contribution to journalEditorialpeer-review

FIT for Purpose: PNA-Based Probes Enable mRNA Imaging in Living Cells
Tilsner, J. & Flors, C., 2 May 2011, In: ChemBioChem. 12, 7, p. 1007-1009 3 p.
Research output: Contribution to journalEditorialpeer-review

Imaging the early events of TMV infection
Christensen, N., Tilsner, J., Lacomme, C. & Oparka, K., Jul 2008, In: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 150, 3, p. S193-S193 1 p.
Research output: Contribution to journalAbstractpeer-review

Molecular characterisation of dormancy phase transition in raspberry (Rubus idaeus L.) buds
Mazzitelli, L., Pont, S. D. A., Walker, P. G., Haupt, S., Tilsner, J., Taylor, M. A., Hedley, P., McNicol, J. W., Hancock, R. D. & Viola, R., Jul 2005, In: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology. 141, 3, p. S291-S291 1 p.
Research output: Contribution to journalAbstractpeer-review

 



Contact

Please contact us if you interested in joining the group!

Dr Jens Tilsner
RM306, BMS Building
North Haugh
St Andrews, KY16 9ST
[email protected]



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