The MacNeill Lab

Wednesday 7 September 2022

Research Centre:

Group Highlights

Research in the MacNeill lab is primarily focused on dissecting the molecular biology of DNA replication and genome stability using two contrasting genetically-tractable model systems, the eukaryotic fission yeast Schizosaccharomyces pombe and the halophilic euryarchaeon Haloferax volcanii. We are also developing genome engineering methods for bacteriophage T5 and collaborate to study the biology of fructan biosynthesis in haloarchaea.

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Research

Chromosomal DNA replication requires the complex interplay of a large number of essential and non-essential protein factors in a temporally- and spatially-coordinated manner. Determining how these factors act together to replicate the genome is central to understanding how the integrity of the genome is maintained within, and across, generations and how genetic diseases such as cancer in humans are avoided. The components of the replication machinery are also potential targets for anti-proliferative drugs and can be used as diagnostic markers for the proliferative state.

The complexity of the replication machinery favours the use of simple model systems to dissect problems of protein structure, function and regulation. Indeed, much of what we know about the eukaryotic replication apparatus has come from model system studies. In the MacNeill lab, research is primarily focused on dissecting the molecular biology of chromosomal DNA replication and genome stability using two contrasting genetically-tractable model systems, the eukaryotic fission yeast Schizosaccharomyces pombe and the halophilic archaeon Haloferax volcanii. In addition to this, we also study the replication machinery of the parasitic protozoan Trypanosoma brucei and in the T5-like bacteriophages (Demerecviridae). We use a variety of methods to address questions of protein structure and function within the chromosome replication apparatus, including genetics and molecular biology, biochemistry, structural biology and bioinformatics.

Chromosomal DNA replication in eukaryotes

DNA polymerase delta plays essential roles in eukaryotic chromosomal DNA replication and also in various DNA repair pathways. We are interested in understanding how Pol delta activity in regulated in vivo, in part using the fission yeast Schizosacharomyces pombe as a model system. We are investigating how post-translational modifications affect Pol delta function in vivo, with particular emphasis on modification of the PolD3 and PolD4 subunits of the complex. We have also expressed and purified the four-subunit Pol delta complex from the thermophilic ascomycete fungus Chaetomium thermophilum (Ct) and have determined its structure by cryo-EM in collaboration with Dr Ramasubramanian Sundaramoorthy (University of Dundee, work in progress). We have also used X-ray crystallography to obtain an understanding of the Chaetomium thermophilum PolD4–PCNA interaction at atomic resolution (collaboration with Dr Magnus Alphey, University of St Andrews).

Chromosomal DNA replication and repair in archaea

Archaea constitute the third domain of life on Earth, comprise an estimated 20% of the planet’s biomass and make major impacts on both biosphere and atmosphere. We are interested in understanding the enzymes and mechanisms of chromosomal DNA replication and repair in archaeal cells and in what this can tell us about the fundamental processes underlying the maintenance of genetic integrity in all cells. We use the highly tractable halophilic (salt-loving) archaeal organisms Haloferax volcanii and Haloarcula hispanica as model systems as this provides us with a range of molecular genetic tools for functional analysis in vivo. Previously we have studied single-stranded DNA binding proteins, the sliding clamp PCNA, ATP- and NAD-dependent DNA ligases, MCM helicase and an entirely novel DNA repair factor named NreA. We are currently investigating the cellular functions of the four Haloferax volcanii RecJ-like proteins to gain fundamental insights into how these proteins have evolved to safeguard the integrity of the archaeal genome.

Molecular biology of bacteriophage T5 replication

The T5-like bacteriophages (Demerecviridae) are a family of lytic bacteriophages that infect Gram-negative bacteria. The phage particles consist of an icosahedral capsid, a lengthy non-contractile tail and a double-stranded DNA genome with the capacity to encode ~160-180 proteins. A number of these proteins have been identified as being essential for phage DNA replication including a DNA polymerase, primase, DNA ligase and exonuclease. We are interested in understanding the function of the remaining essential proteins and understanding how these interact with one another to facilitate rapid replication of the phage genome during infection. We are characterising individual phage T5 proteins biochemically while at the same time developing genome editing methods to allow us to investigate in vivo function in greater detail.

DNA ligases for biotech applications

DNA ligases are essential enzymes in all forms of life and are a cornerstone of recombinant DNA technology. The market leader New England Biolabs alone markets six different ligases in 15 different formulations. In this project, we are exploring the outer limits of DNA ligase sequence space to identify novel enzymes with enhanced properties with commercial potential. Building on recent phylogenetic analysis, we are focusing on highly diverged and previously unstudied ATP-dependent ligase enzymes encoded by crAss-like phages present in the human gut, by viruses that infect unicellular green algae and amoeba, and by early-branching kinetoplastids. Ligase proteins are being expressed and purified in recombinant form and the purified enzymes tested for stability and activity on different substrates under a range of conditions.

Group Members

Stuart MacNeill
Group leader

Abdul Basit
Postdoctoral researcher, 2022-2024

Anna Zolotariof
PhD student, 2020-2024

Uma Maheshwari
MSc(Res) student, 2022-2023

Polina Foteva
Honours project student, 2022-2023

Campbell Wolford
Honours project student, 2022-2023

Tallulah
Lab goldfish, currently on sabbatical

Publications

Books

The eukaryotic replisome: a guide to protein structure and function
MacNeill, S. (ed.), 2012, Springer Science and Business Media.
Research output: Book/Report › Book

Chapters

Single-stranded DNA-binding proteins in the Archaea
Taib, N., Gribaldo, S. & MacNeill, S. A., 14 Apr 2021, Single stranded DNA binding proteins. Oliveira, M. T. (ed.). New York, NY: Humana Press/Springer, p. 23-47 25 p. (Methods in molecular biology; vol. 2281).
Research output: Chapter in Book/Report/Conference proceeding › Chapter

Composition and dynamics of the eukaryotic replisome: a brief overview
MacNeill, S., 2012, The eukaryotic replisome: a guide to protein structure and function. MacNeill, S. (ed.). Dordrecht: Springer, p. 1-17 17 p. (Subcellular Biochemistry; vol. 62).
Research output: Chapter in Book/Report/Conference proceeding › Chapter

Chromosomal DNA replication in yeast: enzymes and mechanisms
MacNeill, S. A. & Burgers, PMJ., 2000, Frontiers in Molecular Biology: The Yeast Nucleus. IRL Press, p. 19-57
Research output: Chapter in Book/Report/Conference proceeding › Chapter

Cell cycle control in fission yeast
MacNeill, S. A. & Nurse, P., 1997, The Molecular And Cellular Biology Of Yeast. Cold Spring Harbor Press, p. 697-763
Research output: Chapter in Book/Report/Conference proceeding › Chapter

Control of mitosis in fission yeast
MacNeill, S. A. & Fantes, PA., 1995, Frontiers in Molecular Biology: The Cell Cycle. IRL Press, p. 63-105
Research output: Chapter in Book/Report/Conference proceeding › Chapter

Methods for analysis of the fission yeast cell cycle
MacNeill, S. A. & Fantes, PA., 1994, The Cell Cycle: A Practical Approach. IRL Press, p. 93-125
Research output: Chapter in Book/Report/Conference proceeding › Chapter

Molecular mechanisms of cell cycle control in yeast
MacNeill, S. A., 1994, The Mycota: Growth, Differentiation and Sexuality. Spinger-Verlag, p. 3-23
Research output: Chapter in Book/Report/Conference proceeding › Chapter

Articles

Biogenesis, inheritance and 3D ultrastructure of the microsporidian mitosome
Hacker, C., Sendra, K., Keisham, P. D., Filipescu, T., Lucocq, J., Salimi, F., Ferguson, S. G. A., Bhella, D., MacNeill, S., Embley, M. & Lucocq, J. M., 1 Jan 2024, In: Life Science Alliance. 7, 1, 23 p., e202201635.
Research output: Contribution to journal › Article › peer-review

Canonical binding of Chaetomium thermophilum DNA polymerase δ/ζ subunit PolD3 and flap endonuclease Fen1 to PCNA
Alphey, M. S., Wolford, C. & MacNeill, S., 18 Dec 2023, In: Frontiers in Molecular Biosciences. 10, 9 p., 1320648.
Research output: Contribution to journal › Article › peer-review

Non‐canonical binding of the Chaetomium thermophilum PolD4 N‐terminal PIP motif to PCNA involves Q‐pocket and compact 2‐fork plug interactions but no 310 helix
Yang, D., Alphey, M. S. & MacNeill, S., 22 Aug 2022, (E-pub ahead of print) In: FEBS Journal. Early View, 14 p., 16590.
Research output: Contribution to journal › Article › peer-review

Remote homology detection identifies a eukaryotic RPA DBD-C-like DNA binding domain as a conserved feature of archaeal Rpa1-like proteins
MacNeill, S., 20 Jul 2021, In: Frontiers in Molecular Biosciences. 8, 8 p., 675229.
Research output: Contribution to journal › Article › peer-review

Unexpected insertion of carrier DNA sequences into the fission yeast genome during CRISPR–Cas9 mediated gene deletion
Longmuir, S., Akhtar, N. & MacNeill, S. A., 29 Mar 2019, In: BMC Research Notes. 12, 5 p., 191.
Research output: Contribution to journal › Article › peer-review

Branched late-steps of the cytosolic iron-sulphur cluster assembly machinery of Trypanosoma brucei
Tonini, M. L., Peña-Diaz, P., Haindrich, A. C., Basu, S., Kriegová, E., Pierik, A. J., Lill, R., MacNeill, S. A., Smith, T. K. & Lukeš, J., 22 Oct 2018, In: PLoS Pathogens. 14, 10, 31 p., e1007326.
Research output: Contribution to journal › Article › peer-review

Insights into the evolutionary conserved regulation of Rio ATPase activity
Knüppel, R., Christensen, R., Gray, F. C., Esser, D., Strauss, D., Medenbach, J., Siebers, B., MacNeill, S. A., LaRonde, N. & Ferreira-Cerca, S., 16 Feb 2018, In: Nucleic Acids Research. 46, 3, p. 1441-1456 16 p.
Research output: Contribution to journal › Article › peer-review

Screening of the MMV and GSK open access chemical boxes using a viability assay developed against the kinetoplastid Crithidia fasciculata
Kipandula, W., Young, S. A., MacNeill, S. A. & Smith, T. K., Jun 2018, In: Molecular and Biochemical Parasitology. 222, p. 61-69 9 p.
Research output: Contribution to journal › Article › peer-review

The archaeal RecJ-like proteins: nucleases and ex-nucleases with diverse roles in replication and repair
MacNeill, S. A., Dec 2018, In: Emerging Topics in Life Sciences. 2, 4, p. 493-501
Research output: Contribution to journal › Review article › peer-review

Tandem affinity purification of exosome and replication factor C complexes from the non-human infectious kinetoplastid parasite Crithidia fasciculata
Kipandula, W., Smith, T. K. & MacNeill, S. A., Oct 2017, In: Molecular and Biochemical Parasitology. 217, p. 19-22 4 p.
Research output: Contribution to journal › Article › peer-review

Meet the neighbours: mapping local protein interactomes by proximity-dependent labelling with BioID
Varnaitė, R. & MacNeill, S. A., 4 Oct 2016, In: Proteomics. 16, 19, p. 2503-2518 16 p.
Research output: Contribution to journal › Review article › peer-review

PCNA-binding proteins in the archaea: novel functionality beyond the conserved core
MacNeill, S. A., Aug 2016, In: Current Genetics. 62, 3, p. 527-532 6 p.
Research output: Contribution to journal › Review article › peer-review

A novel archaeal DNA repair factor that acts with the UvrABC system to repair mitomycin C-induced DNA damage in a PCNA-dependent manner
Giroux, X. & MacNeill, S., 22 Dec 2015, In: Molecular Microbiology. 99, 1, p. 1-14
Research output: Contribution to journal › Article › peer-review

Inhibiting NAD+-dependent DNA ligase activity with 2-(cyclopentyloxy)-5'-deoxyadenosine (CPOdA) offers a new tool for DNA replication and repair studies in the model archaeon Haloferax volcanii
Giroux, X. & MacNeill, S., 2015, In: FEMS Microbiology Letters. 362, 21, 5 p., fnv181.
Research output: Contribution to journal › Article › peer-review

Molecular genetic methods to study DNA replication protein function in Haloferax volcanii, a model archaeal organism.
Giroux, X. & MacNeill, S., 2015, In: Methods in Molecular Biology. 1300, p. 187-218 31 p.
Research output: Contribution to journal › Article › peer-review

Identification of a candidate Rad1 subunit for the kinetoplastid 9-1-1 (Rad9-Hus1-Rad1) complex
MacNeill, S., 19 Dec 2014, In: Biology. 3, 4, p. 922-927 6 p.
Research output: Contribution to journal › Article › peer-review

The haloarchaeal MCM proteins: bioinformatic analysis and targeted mutagenesis of the β7-β8 and β9-β10 hairpin loops and conserved zinc binding domain cysteines
Kristensen, T. P., Cherian, R. M., Gray, F. C. & MacNeill, S., 26 Mar 2014, In: Frontiers in Microbiology. 5, 123.
Research output: Contribution to journal › Article › peer-review

Structure and evolutionary origins of the CMG complex
Onesti, S. & MacNeill, S., Mar 2013, In: Chromosoma. 122, 1-2, p. 47-53
Research output: Contribution to journal › Review article › peer-review

Conditional inactivation of replication proteins in fission yeast using hormone-binding domains
Pai, C-C., Schnick, J., MacNeill, S. & Kearsey, S., 2012, In: Methods.
Research output: Contribution to journal › Article › peer-review

Identification of essential and non-essential single-stranded DNA-binding proteins in a model archaeal organism
Skowyra, A. & MacNeill, S., 1 Feb 2012, In: Nucleic Acids Research. 40, 3, p. 1077-1090 14 p.
Research output: Contribution to journal › Article › peer-review

Ribonucleotide reductase activity is coupled to DNA synthesis via proliferating cell nuclear antigen
Salguero, I., Guarino, E., Shepherd, M. E. A., Deegan, T. D., Havens, C. G., MacNeill, S. A., Walter, J. C. & Kearsey, S. E., 24 Apr 2012, In: Current Biology. 22, 8, p. 720-726 7 p.
Research output: Contribution to journal › Article › peer-review

Protein-protein interactions in the archaeal core replisome
MacNeill, S. A., Feb 2011, In: Biochemical Society Transactions. 39, p. 163-168 6 p.
Research output: Contribution to journal › Article › peer-review

Purification and functional inactivation of the fission yeast MCMMCM-BP complex
Li, J., Schnick, J., Hayles, J. & MacNeill, S. A., 15 Dec 2011, In: FEBS Letters. 585, 24, p. 3850-3855 6 p.
Research output: Contribution to journal › Article › peer-review

Structure and function of the GINS complex, a key component of the eukaryotic replisome.
MacNeill, S. A., 15 Jan 2010, In: Biochemical Journal. 425, 3, p. 489-500 12 p.
Research output: Contribution to journal › Review article › peer-review

The archaeo-eukaryotic GINS proteins and the archaeal primase catalytic subunit PriS share a common domain
Swiatek, A. & MacNeill, S. A., 12 Apr 2010, In: Biology Direct. 5, 1, p. 17 6 p.
Research output: Contribution to journal › Article › peer-review

The eukaryotic replicative DNA polymerases take shape
Johansson, E. & MacNeill, S. A., Jun 2010, In: Trends in Biochemical Sciences. 35, 6, p. 339-347 9 p.
Research output: Contribution to journal › Review article › peer-review

Functional mapping of the fission yeast DNA polymerase delta B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis
Garcia, JS., Baranovskiy, AG., Knatko, EV., Gray, FC., Tahirov, TH. & MacNeill, S. A., 17 Aug 2009, In: BMC Molecular Biology. 10, 13 p., 82.
Research output: Contribution to journal › Article › peer-review

GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast
Pai, C. C., Garcia, I., Wang, S. W., Cotterill, S., MacNeill, S. A. & Kearsey, S. E., 15 Feb 2009, In: Molecular Biology of the Cell. 20, 4, p. 1213-1222 10 p.
Research output: Contribution to journal › Article › peer-review

Inactivating pentapeptide insertions in the fission yeast replication factor C subunit Rfc2 cluster near the ATP-binding site and arginine finger motif
Gray, FC., Whitehead, KA. & MacNeill, S. A., Sept 2009, In: FEBS Journal. 276, 17, p. 4803-4813 11 p.
Research output: Contribution to journal › Article › peer-review

MCM-GINS and MCM-MCM interactions in vivo visualised by bimolecular fluorescence complementation in fission yeast
Akman, G. & MacNeill, S. A., 19 Feb 2009, In: BMC Cell Biology. 10, 7 p., 12.
Research output: Contribution to journal › Article › peer-review

Structural insights into the adaptation of proliferating cell nuclear antigen (PCNA) from Haloferax volcanii to a high-salt environment
Morgunova, E., Gray, FC., MacNeill, S. A. & Ladenstein, R., Oct 2009, In: Acta Crystallographica. Section D, Biological crystallography. 65, p. 1081-1088 8 p.
Research output: Contribution to journal › Article › peer-review

The haloarchaeal chromosome replication machinery
MacNeill, S., Feb 2009, In: Biochemical Society Transactions. 37, 1, p. 108-113
Research output: Contribution to journal › Review article › peer-review

Rapid regulation of protein activity in fission yeast
Boe, C. A., Garcia, I., Pai, C-C., Sharom, J. R., Skjolberg, H. C., Boye, E., Kearsey, S., MacNeill, S. A., Tyers, M. D. & Grallert, B., 5 May 2008, In: BMC Cell Biology. 9, 11 p., 23.
Research output: Contribution to journal › Article › peer-review

ATP-dependent and NAD+-dependent DNA ligases share an essential function in the halophilic archaeon Haloferax volcanii
Zhao, A., Gray, FC. & MacNeill, S. A., Feb 2006, In: Molecular Microbiology. 59, 3, p. 743-752 10 p.
Research output: Contribution to journal › Article › peer-review

Biochemical characterisation of LigN, an NAD+-dependent DNA ligase from the halophilic euryarchaeon Haloferax volcanii that displays maximal in vitro activity at high salt concentrations
Poidevin, L. & MacNeill, S. A., Nov 2006, In: BMC Molecular Biology. 7
Research output: Contribution to journal › Article › peer-review

Contrasting effects of Elg1-RFC and Ctf18-RFC inactivation in the absence of fully functional RFC in fission yeast
Kim, J., Robertson, K., Mylonas, KJL., Gray, FC., Charapitsa, I. & MacNeill, S. A., 2005, In: Nucleic Acids Research. 33, 13, p. 4078-4089 12 p.
Research output: Contribution to journal › Article › peer-review

Genome stability: A self-sufficient DNA repair machine
MacNeill, S. A., 11 Jan 2005, In: Current Biology. 15, 1, p. R21-23 3 p.
Research output: Contribution to journal › Review article

Functional analysis of subcellular localization and protein-protein interaction sequences in the essential DNA ligase I protein of fission yeast
Martin, IV. & MacNeill, S. A., Jan 2004, In: Nucleic Acids Research. 32, 2, p. 632-642 11 p.
Research output: Contribution to journal › Article › peer-review

Genetic and biochemical analyses of Pfh1 DNA helicase function in fission yeast
Ryu, GH., Tanaka, H., Kim, DH., Kim, JH., Bae, SH., Kwon, YN., Rhee, JS., MacNeill, S. A. & Seo, YS., Aug 2004, In: Nucleic Acids Research. 32, 14, p. 4205-4216 12 p.
Research output: Contribution to journal › Article › peer-review

Genetics of lagging strand DNA synthesis and maturation in fission yeast: suppression analysis links the Dna2-Cdc24 complex to DNA polymerase delta
Tanaka, H., Ryu, GH., Seo, YS. & MacNeill, S. A., 2004, In: Nucleic Acids Research. 32, 21, p. 6367-6377 11 p.
Research output: Contribution to journal › Article › peer-review

Mapping and mutation of the conserved DNA polymerase interaction motif (DPIM) located in the C-terminal domain of fission yeast DNA polymerase delta subunit Cdc27
Gray, FC., Pohler, JRG., Warbrick, E. & MacNeill, S. A., Dec 2004, In: BMC Molecular Biology. 5, 21.
Research output: Contribution to journal › Article › peer-review

The C-terminal zinc finger of the catalytic subunit of DNA polymerase delta is responsible for direct interaction with the B-subunit
Garcia, JS., Ciufo, LF., Yang, XW., Kearsey, SE. & MacNeill, S. A., Jun 2004, In: Nucleic Acids Research. 32, 10, p. 3005-3016 12 p.
Research output: Contribution to journal › Article › peer-review

Genome stability: A new member of the RFC family
Kim, JY. & MacNeill, S. A., 11 Nov 2003, In: Current Biology. 13, 22, p. R873-875 3 p.
Research output: Contribution to journal › Review article › peer-review

ATP-dependent DNA ligases
Martin, I. V. & MacNeill, S. A., 2002, In: Genome Biology. 3, 4, p. - 7 p.
Research output: Contribution to journal › Review article › peer-review

Genome sequencing: And then there were six
MacNeill, S. A., 16 Apr 2002, In: Current Biology. 12, 8, p. R294-296 3 p.
Research output: Contribution to journal › Review article › peer-review

The fission yeast pfh1 gene encodes an essential 5' to 3' DNA helicase required for the completion of S-phase
Tanaka, H., Ryu, GH., Seo, YS., Tanaka, K., Okayama, H., MacNeill, S. A. & Yuasa, Y., 1 Nov 2002, In: Nucleic Acids Research. 30, 21, p. 4728-4739 12 p.
Research output: Contribution to journal › Article › peer-review

The influence of the cdc27 subunit on the properties of the Schizosaccharomyces pombe DNA polymerase delta
Bermudez, VP., MacNeill, S. A., Tappin, I. & Hurwitz, J., 27 Sept 2002, In: Journal of Biological Chemistry. 277, 39, p. 36853-36862 10 p.
Research output: Contribution to journal › Article › peer-review

DNA replication: Partners in the Okazaki two-step
MacNeill, S. A., 16 Oct 2001, In: Current Biology. 11, 20, p. R842-R844 3 p.
Research output: Contribution to journal › Article › peer-review

Understanding the enzymology of archaeal DNA replication: progress in form and function
MacNeill, S. A., 1 May 2001, In: Molecular Microbiology. 40, 3, p. 520 529 p.
Research output: Contribution to journal › Review article › peer-review

Essential interaction between the fission yeast DNA polymerase delta subunit Cdc27 and Pcn1 (PCNA) mediated through a C-terminal p21Cip1-like PCNA binding motif
Reynolds, N., Warbrick, E., Fantes, PA. & MacNeill, S. A., 1 Mar 2000, In: EMBO Journal. 19, 5, p. 1108-1118 11 p.
Research output: Contribution to journal › Article › peer-review

Genetic analyses of Schizosaccharomyces pombe dna2+ reveal that Dna2 plays an essential role in Okazaki fragment metabolism
Kang, HY., Choi, E., Bae, SH., Lee, KH., Gim, BS., Kim, HD., Park, C., MacNeill, S. A. & Seo, YS., Jul 2000, In: Genetics. 155, 3, p. 1055-1067 13 p.
Research output: Contribution to journal › Article › peer-review

The Schizosaccharomyces pombe rfc3+ gene encodes a homologue of the human hRFC36 and Saccharomyces cerevisiae Rfc3 subunits of replication factor C
Gray, FC. & MacNeill, S. A., Mar 2000, In: Current Genetics. 37, 3, p. 159-167 9 p.
Research output: Contribution to journal › Article › peer-review

A key role for replication factor C in DNA replication checkpoint function in fission yeast
Reynolds, N., Fantes, PA. & MacNeill, S. A., 15 Jan 1999, In: Nucleic Acids Research. 27, 2, p. 462-469 8 p.
Research output: Contribution to journal › Article › peer-review

Characterisation of XlCdc1, a Xenopus homologue of the small (PolD2) subunit of DNA polymerase delta; identification of ten conserved regions I-X based on protein sequence comparisons across ten eukaryotic species
Reynolds, N. & MacNeill, S. A., 1 Apr 1999, In: Gene. 230, 1, p. 15-22 8 p.
Research output: Contribution to journal › Article › peer-review

Cdm1, the smallest subunit of DNA polymerase delta in the fission yeast Schizosaccharomyces pombe, is non-essential for growth and division
Reynolds, N., Watt, A., Fantes, PA. & MacNeill, S. A., Oct 1998, In: Current Genetics. 34, 4, p. 250-258 9 p.
Research output: Contribution to journal › Article › peer-review

DNA polymerase delta isolated from Schizosaccharomyces pombe contains five subunits
Zuo, SJ., Gibbs, E., Kelman, Z., Wang, TSF., ODonnell, M., MacNeill, S. A. & Hurwitz, J., 14 Oct 1997, In: Proceedings of the National Academy of Sciences of the United States of America. 94, 21, p. 11244-11249 6 p.
Research output: Contribution to journal › Article › peer-review

Genetic and physiological analysis of DNA replication in fission yeast
MacNeill, S. A. & Fantes, PA., 1997, In: Methods In Enzymology. 283, p. 440-459 20 p.
Research output: Contribution to journal › Article › peer-review

The fission yeast Cdc1 protein, a homologue of the small subunit of DNA polymerase delta, binds to Pol3 and Cdc27
MacNeill, S. A., Moreno, S., Reynolds, N., Nurse, P. & Fantes, PA., 2 Sept 1996, In: EMBO Journal. 15, 17, p. 4613-4628 16 p.
Research output: Contribution to journal › Article › peer-review

Meiosis: New roles for RNA in fission yeast
MacNeill, S. & Fantes, P., 1 Jan 1995, In: Current Biology. 5, 1, p. 4-6 3 p.
Research output: Contribution to journal › Article › peer-review

Genetic analysis of human p34cdc2 function in fission yeast
MacNeill, S. A. & Nurse, P., Sept 1993, In: Molecular and General Genetics. 240, 3, p. 315-322 8 p.
Research output: Contribution to journal › Article › peer-review

Mutational analysis of the fission yeast p34cdc2 protein kinase gene
MacNeill, S. A. & Nurse, P., Jan 1993, In: Molecular and General Genetics. 236, 2-3, p. 415-426 12 p.
Research output: Contribution to journal › Article › peer-review

Cold-sensitive mutants of p34cdc2 that suppress a mitotic catastrophe phenotype in fission yeast
AYSCOUGH, K., HAYLES, J., MacNeill, S. A. & NURSE, P., Apr 1992, In: Molecular and General Genetics. 232, 3, p. 344-350 7 p.
Research output: Contribution to journal › Article › peer-review

Molecular cloning and sequence analysis of cdc27+ required for the G2-M transition in the fission yeast Schizosaccharomyces pombe
HUGHES, DA., MacNeill, S. A. & FANTES, PA., Feb 1992, In: Molecular and General Genetics. 231, 3, p. 401-410 10 p.
Research output: Contribution to journal › Article › peer-review

Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2
Paris, J., Le Guellec, R., Couturier, A., Le Guellec, K., Omilli, F., Camonis, J., MacNeill, S. A. & Philippe, M., Feb 1991, In: Proceedings of the National Academy of Sciences of the United States of America. 88, 3, p. 1039-1043 5 p.
Research output: Contribution to journal › Article › peer-review

Controlling cell cycle progress in the fission yeast Schizosaccharomyces pombe
MacNeill, S. A., Warbrick, E. & Fantes, P. A., 1 Jan 1991, In: Current Opinion in Genetics and Development. 1, 3, p. 307-312 6 p.
Research output: Contribution to journal › Article › peer-review

Isolation, characterization and molecular cloning of new mutant alleles of the fission yeast p34cdc2 protein kinase gene - identification of temperature-sensitive G2-arresting alleles
MacNeill, S. A., CREANOR, J. & NURSE, P., Sept 1991, In: Molecular and General Genetics. 229, 1, p. 109-118 10 p.
Research output: Contribution to journal › Article › peer-review

New elements in the mitotic control of the fission yeast Schizosaccharomyces pombe
FANTES, PA., WARBRICK, E., HUGHES, DA. & MacNeill, S. A., 1991, In: Cold Spring Harbor Symposia on Quantitative Biology. 56, p. 605-611
Research output: Contribution to journal › Article › peer-review

p68 RNA helicase - identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts
Iggo, RD., Jamieson, DJ., MacNeill, S. A., Southgate, J., McPheat, J. & Lane, DP., Mar 1991, In: Molecular and Cellular Biology. 11, 3, p. 1326-1333 8 p.
Research output: Contribution to journal › Article › peer-review

Molecular cloning and sequence-analysis of mutant alleles of the fission yeast cdc2 protein kinase gene: implications for cdc2 protein-structure and function
CARR, AM., MacNeill, S. A., HAYLES, J. & NURSE, P., Jul 1989, In: Molecular and General Genetics. 218, 1, p. 41-49 9 p.
Research output: Contribution to journal › Article › peer-review

Cell cycle regulation in yeasts and man: Towards a unifying mechanism
Simanis, V., Carr, A. M., Goss, M., Lee, M. G., Macneill, S. A. & Nurse, P., 1 Sept 1987, In: Antonie van Leeuwenhoek. 53, 5, p. 319-323 5 p.
Research output: Contribution to journal › Article › peer-review

Other publications

Inside Front Cover: Meet the neighbors: Mapping local protein interactomes by proximity-dependent labeling with BioID
Varnaitė, R. & MacNeill, S. A., Oct 2016, In: Proteomics. 16, 19
Research output: Contribution to journal › Comment/debate › peer-review

A unified nomenclature for the subunits of eukaryotic DNA polymerase delta
MacNeill, S. A., Baldacci, G., Burgers, PM. & Hubscher, U., 1 Jan 2001, In: Trends in Biochemical Sciences. 26, 1, p. 16 17 p.
Research output: Contribution to journal › Letter

Genetic interactions in the control of mitosis in fission yeast
MacNeill, S. A. & NURSE, P., Jul 1989
Research output: Other contribution

Contact

Dr Stuart MacNeill
School of Biology
Biomedical Sciences Research Complex
University of St Andrews
St Andrews
KY16 9ST
UK

T: +44 (0)1334 46 72 68
E: [email protected]

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