Molecular-scale optical imaging tools for decoding the structural basis of cellular signalling
Dr Izzy Jayasinghe, University of Sheffield
25 October 2022, 1.00pm, MS Teams
In this seminar, I will outline how the continued improvement and strategic adaptation of super-resolution microscopy tools have enhanced our understanding of the nanoscale structures underpinning life. I will illustrate some of these advances with examples where super-resolution modalities such as STORM, DNA-PAINT and Expansion Microscopy have allowed us to better visualise ryanodine receptor (RyR) and IP3-receptor nanodomains in tissues like the heart, muscle and neural tissues. Correlative structure-function imaging has allowed us to resolve, count and identify the ion channel clustering patterns which spatially encode the fast calcium signals within the cells.